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KMID : 1094720090140040513
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 4 p.513 ~ p.522
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Extracellular Ligninolytic Enzymes by Lentinus polychrous L?. under Solid-state Fermentation of Potential Agro-industrial Wastes and Their Effectiveness in Decolorization of Synthetic Dyes
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Sarnthima Rakrudee
Khammuang Saranyu
Svasti Jisnuson
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Abstract
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Six agro-industrial wastes were evaluated as a support for ligninolytic enzyme production by the white-rot fungus Lentinus polychrous L?. under solid-state fermentation. Enzyme production was markedly different according to the substrate used. Rice bran (RB) yielded the highest laccase activity of 1,449 U/L (after 21 days of culture) with specific activity of 4.4 U/g substrate. Rice bran supplemented with rice husk (RH) (2:1 by wt) showed high laccase activity of 1,425 U/L with specific activity of 10.0 U/g substrate (after 17 days of culture). The crude enzyme of the RH-RB culture also contained manganese peroxidase (MnP) and manganese-independent peroxidase (MIP) activities in relative proportions of 1.9:1.4:1 of laccase:MnP:MIP, respectively. Zymogram studies showed the same isoenzyme pattern with these ligninolytic enzymes. The high enzyme production level and low substrate cost of SSF-L. polychrous L?. suggest that it has potential for industrial applications. Our studies showed that the crude enzyme from this culture exhibited in vitro decolorization of Indigo Carmine. The highest efficiency of dye decolorization was observed under alkaline conditions (pH 9.0) at an initial dye concentration of 10 mg/L. The rather high pH conditions and high efficiency in Indigo Carmine decolorization make the enzyme further interest for the applications in treatment of waste water from the textile industry, which contains synthetic dyes.
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KEYWORD
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laccase, ligninolytic enzyme, Lentinus polychrous L?., solid-state fermentation, agricultural wastes, decolorization
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